Which one of the following statements is not true regarding gel electrophoresis technique?

Options

1. The separated DNA fragments are stained by ethidium bromide
2. The presence of chromogenic substrate gives blue coloured DNA bands
3. Bright orange bands can be seen under UV light
4. The process of extraction of separated DNA strands from gel is called elution

Correct Answer

The presence of chromogenic substrate gives blue coloured DNA bands

Explanation

Gel electrophoresis uses ethidium bromide to stain DNA which fluoresces orange under UV light. Chromogenic substrates are used in enzyme assays, not DNA staining.

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