Plasmid pBR322 has PstI site within ampR gene. If a gene is inserted here, what happens?

Options

1. Resists ampicillin and produces β-galactoside
2. Lysis of host cell
3. Novel protein with dual function
4. Loses ampicillin resistance

Correct Answer

Loses ampicillin resistance

Explanation

Insertion in the ampR gene disrupts its function, so the recombinant plasmid cannot confer ampicillin resistance.

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